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KMID : 1094720080130010108
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Biotechnology and Bioprocess Engineering
2008 Volume.13 No. 1 p.108 ~ p.114
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Optimized production of lignolytic manganese peroxidase in immobilized cultures of Phanerochaete chrysosporium
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Kwon Hyuk-Sung
Chung Eun-Yong
Oh Jun-Se
Lee Chang-Ha
Ahn Ik-Sung
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Abstract
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Manganese peroxidase (MnP) is a key enzyme involved in the lignolysis of white-rot fungi. The purpose of this study is to investigate the effect of immobilization and culture conditions on MnP production in cultures of Phanerochaete chrysosporium grown on polyurethane foam. Higher concentrations of foam and lower levels of spore inoculums resulted in the formation of scattered mycelial pellets, increased autolysis of chlamydospore-like cells (a reservoir of MnP), and a higher activity of MnP. Even though MnP was a secondary metabolite, the addition of 5 times more glucose and diammonium tartrate, as carbon and nitrogen sources, resulted in a 4 fold increase in the dry cell mass. However, MnP activity decreased under these conditions to less than half, due to the formation of increasingly dense pellets and the inhibited lysis of chlamydospore-like cells.
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KEYWORD
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manganese peroxidase, immobilization, Phanerochaete chrysosporium chlamydospore-like cell, autolysis
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